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Immunoprecipitation assay

QS Qian-Qian Sha
XD Xing-Xing Dai
JJ Jun-Chao Jiang
CY Chao Yu
YJ Yu Jiang
JL Junping Liu
XO Xiang-Hong Ou
SZ Song-Ying Zhang
HF Heng-Yu Fan
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The immunoprecipitation (ChIP) assay in HeLa cells was performed using the SimpleChIP Enzymatic Chromatin IP Kit (CST, 9003) according to the manufacturer’s protocol. Approximately 4 × 106 cells were used for each immunoprecipitation. Immunoprecipitation of immunoglobulin G (CST#2729; 1 µg, as a negative control) or HA (CST#3724; 1 µg per sample) was performed at 48 h after transfection. In all, 10% total DNA was used for input evaluation. PCR products of immunoprecipitated and input samples were analyzed on a 2% agarose gel. Sequences of primers for PCR amplification are provided in Supplementary Table 2.

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