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NO production by microglia cultures was assessed by measuring nitrite accumulation in the culture medium by Griess Reagent Kit according to manufacturer's instructions (Molecular Probes, MA, USA). The absorbance was measured at 570 nm in a spectrophotometer microplate reader (BioTek Instruments Inc., VT, USA).
Copyright and License information: ©2018 Lepore, D'Alessandro, Antonangeli, Santoro, Esposito, Limatola and Trettel.
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