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Electrophoretic mobility shift assay

JM Joshua E Mayfield
SI Seema Irani
EE Edwin E Escobar
ZZ Zhao Zhang
NB Nathaniel T Burkholder
MR Michelle R Robinson
MM M Rachel Mehaffey
SS Sarah N Sipe
WY Wanjie Yang
NP Nicholas A Prescott
KK Karan R Kathuria
ZL Zhijie Liu
JB Jennifer S Brodbelt
YZ Yan Zhang
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SDS-PAGE analysis was performed using 10–15% acrylamide gels containing 1% SDS. GST-CTD samples were prepared by boiling with SDS-PAGE loading dye at 95°C for 5 min. This was also used to quench time-course reactions. A volume containing approximately 1 μg of phosphorylated GST-CTD substrate or no kinase control was loaded into wells and resolved at ~150V for 1 hr at room temperature. Gels were stained with Coomassie Brilliant Blue and visualized on G: BOX imaging systems (Syngene).

Copyright and License information:  ©2019, Mayfield et al
This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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