Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Concise Method
Streptavidin–biotin affinity pull-down assay
Request a Protocol
Ask a
question
Favorite
Cell lysates were harvested and incubated with free biotin or WJ460–biotin at 4 °C with gentle rotation. Recombinant streptavidin agarose beads were subsequently added to precipitate proteins interacting with biotin-labeled WJ460. The bead-bound proteins were separated by SDS-PAGE and visualized by Silver Staining. The indicated protein-containing band was cut out for mass spectrometry.
Copyright and License information: The Author(s) ©2018
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
