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Histone deacetylase (HDAC) activity assay
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HDAC activity was measured using a commercial kit (HDAC-GloTM I/II Assays; Promega). Protein lysates were prepared with 1% NP buffer excluding either EDTA or EGTA to avoid zinc chelation. After immunoprecipitation with anti-HDAC2, the bead-immunocomplex was mixed with HDAC assay substrate and incubated for more than 15 min at room temperature. HDAC activity was detected using a luminometer. The nonspecific IgG-precipitated HDAC activity was regarded as the blank and was subtracted from the basal level.
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