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HUVECs were seeded on 12-well tissue culture plates (wells pre-coated with M13 phage, M13 phage-RGD, and gelatin). After reaching confluency, a scratch wound was created on the cell monolayer with yellow pipette tips. After that, medium was carefully changed to remove debris, and cells were incubated at 37 °C in incubator (5% CO2). The scratched area of each experimental group was imaged with an inverted microscope (Olympus, Japan) at 0, 9, and 18 hours, respectively17.
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