Q-RTPCR for pro-inflammatory cytokine and chemokine mRNA expression

Renal inflammation after IR was also assessed by measuring pro-inflammatory mRNA markers including IL-6, IL-8, intercellular adhesion molecule-1 (ICAM-1), monocyte chemoattractive protein-1 (MCP-1), macrophage inflammatory protein-2 (MIP-2), and tumor necrosis factor-α (TNF-α) quantitative RT-PCR as described previously with primers listed in Table 2 (35, 36). Primer design was based on published GenBank sequences. To confirm equal RNA loading, GAPDH mRNA expression was also measured.

Primers used in quantitative reverse transcription polymerase chain reactions to amplify mouse cDNAs based on published GenBank sequences. Annealing temperatures used for each primer are also provided.