Online supplemental material

Fig. S1 shows the effect of INF2 KD on the percentage of ciliated cells and the size of the cilia, and the effect of a second shRNA to INF2 on tubulin acetylation. Fig. S2 shows the effect of INF2 KO on tubulin acetylation in various cell lines. Fig. S3 shows representative images of the effect of the overexpression of INF2 mutants on tubulin acetylation in control RPE-1 cells and an immunoblot analysis of the expression of mDia1 and FMNL1 proteins in INF2 KD cells. Fig. S4 shows the effect of cytochalasin D on tubulin acetylation in control cells and the effect of MRTF-A Δ-N100 expression on tubulin acetylation in SMIFH2-treated cells. Table S1 shows the sequence of the primers used in the quantitative analysis of the expression of the various mRNAs examined.