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Detection of anti-Toxoplasma IgG antibody using ELISA technique

AT A Teimouri
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Microtiter plates were coated with soluble antigens of T. gondii, RH strain. Sera were added in dilution of 1:100 in PBS followed by incubation and washing. Anti-human IgG conjugated with horseradish peroxidase (HRP; Dako Denmark A/S, Glostrup, Denmark) was added after incubation. After washing, chromogenic substrate orthophenyline-diamidine (OPD) was added and the reaction was stopped by adding sulfuric acid. The optical density was read and recorded by an automated ELISA reader at 490 nm.14

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