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Mice were administered 3 μg of OVA in PBS containing 30% HP-β-CyD and 10 μg of Alexa Fluor®;594-labeled K3 CpG-ODN at the base of the tail. After 24 h, the draining lymph node was collected, and the cells were stained and measured following the procedure above. The percentages of Alexa Fluor®;594+ cells of conventional dendritic cells (CD11c+ Siglec-H−), plasmacytoid dendritic cells (CD11c+ Siglec-H+) and B cells (CD19+) were analyzed by FlowJo software (Tree Star).
Copyright and License information: ©2018 Hayashi, Momota, Kuroda, Kusakabe, Kobari, Makisaka, Ohno, Suzuki, Nakagawa, Lee, Coban, Onodera, Higashi, Motoyama, Ishii and Arima.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
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