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DnaK ATPase activity assays were performed at 37 °C with DnaK (10 µM), AtcJ wild-type or mutant (50 µM), DnaJ (2 µM), and GrpE (2 µM) when indicated, in 25 mM Hepes pH = 7.5, 150 mM KCl, 5 mM MgCl2, 2 mM ATP, using a pyruvate kinase/lactate dehydrogenase enzyme-coupled assay as described before53.
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