Raw reads from Jaffe et al.,19 were available via study accession SRP045638. The 36 paired-end libraries were analysed using the iRAP pipeline (https://github.com/nunofonseca/irap). First, raw reads in the original FASTQ files underwent quality assessment and filtering.60 They were then aligned against the GRCh38 genome reference using Tophat2,61 with the option: ‘–min-intron-length 6’.
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