FACS Analysis

For flow cytometry, cells were labeled with pre-determined optimum concentrations of antibodies at 4°C for 15 min, followed by washing in FACS Stain Buffer (BD Biosciences). For intracellular staining, cells were fixed and permeabilized with Cytofix/Cytoperm (BD Bioscience), and antibodies we added in the presence of BD PermWash buffer. Dead cells were labeled with LIVE/DEAD® yellow dye (Life Technologies, Carlsbad, CA). A BD LSR or LSRII was used for flow cytometry; and data were analyzed using FlowJo V10 software (Treestar, Ashland, OR). Gastric DCs were gated as live CD45^pos/lineage^neg/HLA-DR^high cells. The lineage cocktail contained antibodies to CD3, CD19, and CD56.