All fluorescence lifetime measurements at 640 nm excitation were performed with a FluoTime200 time resolved spectrometer (Picoquant, Berlin, Germany). Decay curves were analyzed using FluoFit 4.4.0.1 software. Measurements of fluorescence lifetimes of dyes with excitation at 400–560 nm were carried out with a MicroTime200 (Picoquant, Berlin, Germany), equipped with a supercontinuum laser SuperK Extreme EXW12 (NKT Photonics, Germany). The setup is attached to an Olympus IX83 with a 60 × /1.2 ultra-plan-apochromat water-immersion objective.
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