Immunohistochemistry was performed after either paraformaldehyde of Carnoy fixative based fixation64. Antibodies used were: anti-H3P (phosphorylated serine 10 on histone H3; Millipore; 09–797; 1:1000 dilution), anti-VC1 (kindly provided by Prof. Hidefumi Orii (check title); 1:10,000 dilution), anti-SMEDWI-1 (kindly provided by Prof. Jochen Rink; 1:500 dilution), anti-SYNORF-1 (3C11; Developmental Studies Hybridoma Bank; 1:50 dilution), anti-acetylated tubulin (Developmental Studies Hybridoma Bank; 1:200 dilution).
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