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Thirty representative CRC samples were stained for HLA-class I (clone HCA2, BIOZOL, Eching, Germany, diluted 1:200) and HLA-class II (Clone M0775, DAKO, Glostrup, Denmark, diluted 1:200). Staining was performed on formalin-fixed, paraffin-embedded tissue sections on a Lab Vision Autostainer (Thermo Scientific), following the manufacturer’s protocols.
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