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A549 cells infected with SFSV were lysed as described above and then processed as described before (90). In brief, 10% native polyacrylamide gels were prerun at 25 mA for 30 min in native running buffer (25 mM Tris, 192 mM glycine, pH 8.3), with 1% deoxycholate added to the cathode buffer. Samples were supplemented with native loading buffer (250 mM Tris-HCl [pH 6.8], 50% glycerol, 1% deoxycholate, 0.5% bromophenol blue), run at 20 mA for the desired duration, and finally transferred to PVDF membranes via semidry blotting.
Copyright and License information: ©2018 Wuerth et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.
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