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Transected HCC cells (1 × 103 cells each well) were added into a 6-well plate and cultured in the incubator. After two weeks incubation, the formed cell colonies were fixed with 4% paraformaldehyde for 30 min and subsequently stained with 0.5% crystal violet (meilunbio, Dalian, China) for 30 min at room temperature.
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