C. elegans strains

The strains VC308 (rab-7(ok511)/mIn1 [mIs14 dpy-10(e128)]), RB662 (apb-3[ok429]), DA1240 (adIs1240[P_eat-4::GFP + lin-15(+)]), CL2006 (dvIs2[pCL12(P_unc-54::Aβ1–42) + pRF4), and CL4176 ((smg-1(cc546ts) I; dvIs27 [P_myo-3::Aβ + rol-6(su1006)) were provided by the Caenorhabditis Genetics Center. Strain BY250 (vtIs7[P_dat-1::GFP)]) was a generous gift from Randy Blakely (Florida Atlantic University), while TU3401 (sid-1(pk3321); uIS69 [P_myo-2::mCherry; P_unc-119::sid-1]) was a gift from Martin Chalfie (Columbia University) (Table 1).

A summary of all the C. elegans strains utilized in this publication

The isogenic strain UA44 (baIn11[P_dat-1::α-syn, P_dat-1::GFP]) expresses α-synuclein and green fluroescent protein (GFP) in the dopaminergic neurons. UA198 (baIn34[P_eat-4::Aβ, P_myo-2::mCherry]; adIs1240[P_eat-4::GFP]) was generated as previously described (13) and expresses GFP and Aβ in the glutamatergic neurons. The following strains were produced by injecting N2 animals with constructs generated by Gateway Cloning (Invitrogen; described in the section below) to overexpress genes of interest from eat-4 or snb-1 promoters: UA322 (baEx189[P_eat-4::hVPs41, P_unc-54::tdTomato]), UA348 (baEx197[P_eat-4::hVPs41 G, P_unc-54::tdTomato]), UA323 (baEx190[P_snb-1::arl-8, P_unc-54::tdTomato]), UA324 (baEx191[P_eat-4::hVPs41 T146P, P_unc-54::tdTomato]), UA325 (baEx192[P_eat-4::hVPs41 A187T, P_unc-54::tdTomato]),UA326 (baEx193[P_snb-1::arl-8Q75L, P_unc-54::tdTomato]), and UA327 (baEx194[P_snb-1::arl-8 T34N, P_unc-54::tdTomato]). The P_eat-4::sid-1 plasmid was co-injected together with either the hVps41 or the arl-8 plasmids into a sid-1 mutant-containing UA198 strain (UA328; see description in following section) to create the following strains: UA329 (baEx195[P_eat-4::hVps41, P_eat-4::sid-1, P_unc-54::tdTomato]; baIn34[P_eat-4::Aβ,P_myo-2::mCherry]; adIs1240[P_eat-4::GFP] ; sid-1(pk3321)), and UA330 (baEx196[P_eat-4::arl-8, P_eat-4::sid-1, P_unc-54::tdTomato]; baIn34[P_eat-4::Aβ,P_myo-2::mCherry]; adIs1240[P_eat-4::GFP]; sid-1(pk3321)) (Table 1).