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To evaluate the breast cancer cell invasion, the experiments were conducted using 24-well invasion chambers with 8-μm pore-size inserts that were coated with Matrigel (Corning). MDA-MB-231 cells (1.5 × 105 cells/well) were plated on the inserts and were co-cultured with M2 macrophages for 24 h at 37°C. The cells that invaded across the inserts were stained with 0.005% crystal violet (Beyotime), followed by counting cells per field of view under a phase-contrast microscope.
Copyright and License information: ©2018 Chen, Zhang, Cao and Zhang.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
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