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Chemical hypoxia induction with cobalt chloride (CoCl2)
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For chemical hypoxia 150,000 Caco-2 cells were seeded in each well in the 6-well plates, incubated for 24 h to allow their adhesion and 100 µM CoCl2 (Sigma-Aldrich; Merck KGaA, Darmstadt, Germany) were added to the medium and were incubated for 0, 3, 6, 24, 48 and 72 h.
Copyright and License information: ©2018-04-10 Calvo-Anguiano et al.
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