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RNA sequencing

FB Farhan Basit
TM Till Mathan
DS David Sancho
IV I. Jolanda M. de Vries
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Total RNA was isolated from CD1c+ mDCs and pDCs using Trizol (Invitrogen, MA, USA). RNA sequencing and read alignment were performed by BGI TECH SOLUTIONS (Hong Kong). Reads were aligned to human genome version 19. RNA sequencing data is deposited at the Gene Expression Omnibus (GEO; accession number: GSE89442). Data was analyzed using the R platform package “edgeR,” version 3.12, to analyze whole transcriptome principal coordinates analysis (using the “plotMDS” command), differential expression analysis, and GO term analysis. Differential expression was determined by fitting a generalized linear model using the “glmFit” command, and significance was determined using the likelihood ratio test provided by the “glmLRT” command (27).

Copyright and License information:  ©2018 Basit, Mathan, Sancho and de Vries.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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