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In vitro drug release

SH Shih-Ting Huang
YW Yi-Ping Wang
YC Yen-Hui Chen
CL Chin-Tarng Lin
WL Wen-Shan Li
HW Han-Chung Wu
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The PTX release profiles from Taxol and lipo-PTX formulations were investigated according to a dialysis bag diffusion method. Briefly, 1 ml Taxol or lipo-PTX (1 mg/ml) was placed in a dialysis tube (molecular weight cut-off 6–8 kDa; 71509-3; Merck KGaA) and immersed into 40 ml release medium (PBS, pH 7.4 or 5.0). The entire system was incubated at 37°C with orbital agitation at 150 rpm. The concentration of PTX remaining in the dialysis tube at predetermined time intervals (0, 0.5, 1, 2, 4, 6, 8, 24, 48, 72, 96 and 168 h) was measured by HPLC.

Copyright and License information:  ©2018-06-21 Huang et al.
This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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