Lactate dehydrogenase cytotoxicity assay

QG Qiang Gao
YG Yunyan Gu
YJ Yanan Jiang
LF Li Fan
ZW Zixiang Wei
HJ Haobin Jin
XY Xirui Yang
LW Lijuan Wang
XL Xuguang Li
ST Sheng Tai
BY Baofeng Yang
YL Yan Liu
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The lactate dehydrogenase (LDH) assay was performed using the LDH Cytotoxicity Assay Kit (A020-2, Nanjing Jiancheng Bioengineering Institute, Nanjing, China) according to the manufacturer’s protocol. The amount of LDH released by cells can be used as an index of cellular damage. The kit contains WST (4-[3-(4-iodophenyl)-2-(4-nitrophenyl)-2H-5-tetrazolio]-1,3-benzene disulfonate) reagent for detection of LDH released from the damaged cells. Reacting with WST, LDH oxidizes lactate to generate NADH, which can be quantified at 450 nm optical density by a ELIASA (Infinite 200 PRO, Tecan).

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