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The middle portion of the left lobe of the liver was excised and fixed in 4% paraformaldehyde at 4°C for ≥24 h. Then, the tissues were paraffin-embedded, cut into 3-µm thick sections, and stained with hematoxylin for 10 min and eosin for 5 min to observe the degree of inflammation and tissue damage by light microscopy. All procedures were performed at 37°C. The ratios of necrotic and inflammatory areas to total areas were calculated using Image-Pro Plus software 6.0 (Media Cybernetics, Rockville, MD, USA).
Copyright and License information: ©2018 Feng et al.
This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
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