Flow Cytometry.

P-selectin expression was detected with FITC-labeled anti-human (10 μg/mL) or anti-murine P-selectin antibody (1:5). GPIIb/IIIa activation was detected by FITC-labeled PAC-1 (25 μg/mL) binding to human platelets and PE-labeled JON/A (1:5) binding to murine platelets. Mitochondrial inner transmembrane potential (ΔΨm) depolarization in human or murine platelets was measured by JC-1 (2 μg/mL). PS exposure of human or murine platelets was detected by FITC-labeled lactadherin (10 μg/mL). Platelets were measured by a flow cytometer (FC 500; Beckman-Coulter).