MTT assay

An MTT assay was carried out to examine cell viability to determine HCC cell proliferation. The cells (5×10³ cells/ml) treated with different transfection were planted in 96-well plates and incubated for 48 h at 37°C with 5% CO₂. Then, we added MTT reagent (20 ml) to each well at 0, 1, 2, 3 and 4 days followed by incubation for another 4 h. Dimethyl sulfoxide (150 ml) was added to dissolve the crystallization. The absorbance value of cells was measured at 490 nm using enzyme-linked immunoassay.