DOI-Induced Head Twitch.

DOI-induced head twitch was conducted as previously described (37). Briefly, male SERT Ala56 mice and their SERT Gly56 littermates treated with either vehicle (saline, 0.1 mL/10 g body weight, intraperitoneally, repeatedly or acutely administered) MW108 (5–10 mg/kg, i.p.) or MW150 (5–10 mg/kg, i.p, repeatedly or acutely administered) underwent treatments of DOI (1.0 mg/kg, i.p.; Sigma Aldrich) dissolved in sterile saline (0.1 mL/10 g body weight). Thirty-four minutes posttreatment, each respective mouse was placed in a large glass beaker (4 L) containing bedding (1 cm) and two independent reviewers blinded to genotype independently counted head twitches over a period of 15 min. Head twitches were quantified and analyzed.