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Measurement of platelet aggregation
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Platelets (108/mL) were preincubated with or without G-Rg3 in 2 mM CaCl2 for 3 min at 37°C and then stimulated by thrombin (0.05 U/mL). The platelet aggregation assay was performed for 5 min using an aggregometer (Chrono-Log Corporation). Platelet aggregation rate (%) was determined as an increase in light transmission. G-Rg3 was dissolved in 0.1% dimethyl sulfoxide (DMSO).
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This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
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