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2.3. Measurement of proinflammatory cytokine levels using ELISA
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Tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and interleukin-1β (IL-1β) levels on the experimental stroke side were quantified using ExCell ELISA kits (ExCell Biology) as per the manufacturer's instructions. In brief, the brain tissue was homogenized in phosphate-buffered solution (PBS, pH 7.4). The protein concentration of each sample was determined using a BCA Protein Assay Kit (Beyotime, Shanghai, China).
Copyright and License information: ©2018 The Korean Society of Ginseng. Publishing services by Elsevier B.V.
This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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