A2780 (50,000 cells/well) and SKOV3 (80,000 cells/ well) cells were cultured in 6-well plates for 18 h before being treated with lentiviral vectors for 8 h. The cells were incubated further in normal culture medium for 48 h, and then, puromycin was added for 3-4 weeks to establish stable cell lines. The cDNA encoding SNAP-29, SNAP-29 (Mut) and LC3 were cloned in pcDNA3.1, pEGFP-N1 or pmCherry-C1 vectors. The cells were transfected with Lip2000 (Invitrogen, 11668019). The siRNA sequences were: SNAP-29, 5'-AGACAGAAAUUGAGGAGCA-3'; Stx17, 5'- CCGAAAGGAUGACCUAGUA-3'; VAMP8, 5'-GCAACAAGACAGAGGAUCU- 3'. The siRNA was delivered into cells with Lipo2000 according to the instructions of the manufacturer.
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