MSC migration into brain

MSC labelled with PKH-26 (red) or CTGB (green), were administered 48 h after the last dose of cisplatin/PBS. Brains were harvested 12 h later. Frozen brain sections were examined by confocal microscopy for presence of labelled MSC. In a separate experiment, MSC were isolated from GFP transgenic mice and administered to cisplatin treated animals as before. The presence of GFP transgenic transcript in the brain was analyzed using qPCR with the forward Primer (AGT GCT TCA GCC GCT ACC), reverse primer (GAA GAT GGT GCG CTC CTG), and transgenic probe (TTC AAG TCC GCC ATG CCC GAA).