For evaluating the effects of antipsychotic medication on gene expression profiles, we converted daily doses of antipsychotics at the time of the biopsy to chlorpromazine equivalents.31 Then, we conducted linear regression analysis for gene expression levels detected by qPCR using the chlorpromazine equivalents as a covariate. Linear regression was also used to evaluate the effect of smoking (using the number of packs of cigarettes smoked per day as an independent variable) on gene expression levels in the qPCR experiments, and on the neuropsychological assessment scores.
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