SDS–PAGE and Western blot analysis

The protein samples were electrophoresed by 15% SDS–PAGE under reducing and non-reducing conditions and the gels were stained with Coomassie Brilliant Blue (Merck, Germany). For Western blotting, the proteins were transferred onto polyvinylidene fluoride (PVDF) membranes. The membranes were blocked in 2% BSA overnight at 4 °C. To identify the recombinant EntP peptide the membranes were incubated for 60 min at room temperature with horseradish peroxidase (HRP)-labelled antibody (Santa Cruz, USA). Finally, the recombinant peptide was visualized by enhanced chemiluminescence detection system (ECL) method according to the manufacturer’s instructions (Amersham Biosciences).