Genome Sequencing and Annotation

The strain A4B-17 was cultivated in MB to the stationary phase. Its genomic DNA was extracted according to the protocols in Molecular Cloning (Sambrook and Russell, 2001). The genome was sequenced using paired-end sequencing technology (HiSeq 2000 system, Illumina, United States). The shotgun library was constructed with a 500 bp-span and a 6,000 bp-span paired-end library. Library was sequenced using MiSeq reagent kit v3 (Illumina, United States). Reads (100 bp) were assembled using SMRT Analysis 2.3.0 (Berlin et al., 2015). The gene prediction was performed using Glimmer v. 3.02 (Delcher et al., 2007), and the gene product function was annotated by BLAST using the NCBI-nr protein and Swiss-Prot databases. The classification of predicted genes and pathways were analyzed using the COGs (Tatusov et al., 2003) and KEGG (Kanehisa et al., 2016) databases.