Flow cytometry.

CD4 (clone RM4-5), Vβ6 (clone RR4-7), Ki-67 (clone B.56), active caspase-3 (clone C92-605), and CD25 (clone 7D4) were all purchased from BD Biosciences. CD90.1 (Thy1.1, clone HIS51) was purchased from eBiosciences. CD3 (clone 145-2C11), CD8 (clone 53-6.7), CD45.1 (clone A20), CD45.2 (clone 104), TCRγδ (clone GL3), CD326 (clone G8.8), CD19 (clone 6D5), CD90.2 (Thy1.2, clone 53-2.1), CD69 (clone H1.2F3), H2D^d (clone 34-2-12), FOXP3 (clone 150D), IL-10 (clone JES5-16E3), IL-17A (clone TC11-8H10.1), IFN-γ (clone XM1.2), and TNF (clone MP6-XT22) were all purchased from BioLegend. Fixation and permeabilization were undertaken for intracellular staining (BD Fix/Perm Kit; BD Biosciences) and nuclear staining (Fix/Perm Kit; eBiosciences) according to the manufacturer’s instructions. The LIVE/DEAD Fixable Aqua Dead Cell Staining Kit (Invitrogen) was utilized to gate viable cells. Cells were acquired on a BD LSRFortessa and analyzed with FlowJo V9 (Treestar). MR1 tetramers were prepared and used as previously described (7, 8, 11, 75).