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2.7. cAMP direct immunoassay

MK M. E. M. S. Khedr
AA A. M. Abdelmotelb
TB T. A. Bedwell
AS A. Shtaya
MA M. N. Alzoubi
MH M. Abu Hilal
SK S. I. Khakoo
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Levels of cAMP in hepatocytes 24 h following cell seeding and at day 3 or 5 following stimulation with 10−6 M VIP treatment were detected using a cAMP direct immunoassay (Abcam) according to the manufacturer's instructions. cAMP concentrations (μM) were determined and corrected to total proteins concentrations in samples (μg).

Copyright and License information:  ©2018 John Wiley & Sons Ltd

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