Aluminum hydroxide (alum) was obtained in the form of Alhydrogel “85” (Brenntag Biosector, Frederikssund, Denmark). The B Class CpG ODN (CpG) of sequence 5' TCG TCG TTT TTC GGT GCT TTT 3' was synthesized with a nuclease-resistant phosphorothioate backbone (Avecia, Milford, MA, USA) as described previously [12].
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