Water quality, blood biochemistry, oxidative stress biomarkers, and hepatic HSPs mRNA expressions were tested using SAS (version 9.2). The differences between the groups in each time intervals were examined by t-test. While the differences among each time intervals within the group were tested to the one-way analysis of variance (ANOVA) followed by Duncan's post-hoc test. Differences were investigated statistically significant at P < 0.05.
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