SCID mouse xenograft studies

Twelve CB-17 strain SCID male mice, purchased from Taconic (Hudson, NY), were housed in a virus- and antigen-free facility supported by the Health Sciences Laboratory Animal Services at the University of Alberta and were cared for in accordance with the Canadian Council on Animal Care guidelines. All experimental protocols involving mice were reviewed and approved by the University of Alberta Health Sciences Animal Welfare Committee. Briefly, 2 million cells of SupM2-RU-EV, SupM2-RU-MYC, and SupM2-RR-EV growing exponentially were injected into both flanks of 4-week-old mice, four mice each group. The tumor sizes were measured twice every week. These animals were sacrificed when a tumor reached 10 mm in the greatest dimension.