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2.3. Identification of miRNA–Target Interactions

HL Haiming Liu
NW Nan Wu
HZ Hao Zhang
YL Yuanning Liu
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MicroRNA sequences were obtained from the TargetScan website (http://www.targetscan.org/) [27]. LncRNA sequences were obtained from the GENCODE v26. miRNA–mRNA interactions were obtained from three reliable online miRNA-target databases: miRWalk 2.0 [28], miRTarBase 6.0 [29], and DIANA-TarBase v7.0 [30]. miRNA–lncRNA interactions were predicted using TargetScan 7.0 [27] with default parameters. After integrating the above methods, a total of 148,459 non-redundant reliable miRNA–mRNA interactions and 29,523 miRNA–lncRNA predicted interactions were obtained. We then selected 1146 reliable DEM–DEMi interactions and 2794 predicted DEL–DEMi interactions for further analysis.

Copyright and License information:  ©2018 by the authors.
Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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