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Cell culture

DW Doreen William
MW Madlin Walther
BS Björn Schneider
ML Michael Linnebacher
CC Carl Friedrich Classen
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The primary GBM cell lines used in this study were previously established from patient individual GBM tissue in our laboratory [16]. Specimen collection was conducted in accordance with the ethics guidelines for the use of human material, approved by the Ethics Committee of the University of Rostock (Reference number: A 2009/34). Cells were cultured in DMEM/Ham’s F12 (Biochrom, Berlin, Germany) supplemented with 10% FCS (PAN-Biotech, Aidenbach, Germany) and 2mM L-Glutamine (Biochrom) and cultivated at 37°C, 5% CO2 and 95% relative humidity.

Copyright and License information:  ©2018 William et al
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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