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Purified rKSHV.219 was UV-irradiated with 150,000 J/cm2 of energy for two rounds. UV-inactivation was verified by spinoculating AD293 cells at 700 rpm for 60 min at 37°C with 8 μg/ul polybrene, and infection media was replaced with complete DMEM. At 48 h post-infection, FACS (LSRII) analysis ensured no GFP-positive cells. rKSHV.219 and UV-rKSHV.219 from the same viral stock were used within the same experiment.
Copyright and License information: ©2018 Rosario, Santiago, Mesri and Verdun.
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