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Lipid staining by Oil O Red or Bodipy.

YC Yong Chen
KI Kenji Ikeda
TY Takeshi Yoneshiro
AS Annarita Scaramozza
KT Kazuki Tajima
QW Qiang Wang
KK Kyeongkyu Kim
KS Kosaku Shinoda
CS Carlos Henrique Sponton
ZB Zachary Brown
AB Andrew Brack
SK Shingo Kajimura
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Before staining, culture medium of the differentiated adipocytes was discarded. Cells were washed once with PBS, fixed in 4% paraformaldehyde for 20 min, and then stained with Oil Red O solution or for 20 min at ambient temperature or with 20 nM Bodipy in PBS for 15 min at 37 °C. Subsequently, cells were washed with PBS before imaging.

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