Five strains of penicillin-resistant S. aureus (SA-24, SA-69, SA-78, SA-85, and SA-91) were isolated from a healthy adult volunteer. These strains were screened by PCR analysis for the presence of the blaZ gene (data not shown); PCR employed the primer pair described in a previous report (Okamoto et al. 1996). Standard S. aureus strain, SA-12732 (NBRC12732) was obtained from the National Institute of Technology and Evaluation Biological Research Center, Chiba, Japan.
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