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Acetylcholinesterase (AChE) activity assay
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The AChE activity was determined in brain tissue by using an AChE assay kit (Sigma Aldrich Co., St Louis, MO, USA) in accordance with the manufacturer’s instructions. After centrifugation at 18,000×g for 5 min, the working reagent was added to the supernatant for 2 min (initial) or 10 min (final). The absorbance at 412 nm was measured and calculated relative to the control group (100%).
Copyright and License information: The Korean Society of Food Science and Technology and Springer Science+Business Media B.V., part of Springer Nature ©2018
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