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For cell apoptosis detection, 200 µl of binding buffer were added to prepare single cell suspension. In total 5 µl of Annexin V-APC and 10 µl of 7-AAD (Thermo Fisher Scientific, Inc.) were mixed in cell suspension and incubated in the dark for 15 min, followed by cell apoptosis detection using flow cytometry (BD Biosciences, Franklin Lakes, NJ, USA). The data were analyzed using FlowJo software (FlowJo LLC, Ashland, OR, USA).
Copyright and License information: ©2018 Lu et al.
This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
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