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Lnk–/– mice were treated with doxycycline (0.44 mg/ml, Sigma-Aldrich) starting 1 week prior to Ang II infusion and continuing during the 2 weeks of Ang II infusion. Mice received a calculated dose of 100 mg/kg/day in the drinking water as previously described (40). Each bottle was protected from the light and fresh doxycycline solution was prepared every 2 days.
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