2.4. Plasmids and stable overexpression and knockdown of PRMT9 in hepatocellular carcinoma cells

The pLV‐Puro‐GFP‐PRMT9 lentiviral vector was purchased from Guangzhou Cyagen Biosciences. The GV248‐GFP‐shRNA‐PRMT9 lentiviral vectors containing shRNA targeting PRMT9 were purchased from Shanghai GeneChem and the hairpin shRNA sequences are described in Document S1. The lentiviruses produced from pLV‐Puro‐GFP‐PRMT9 lentiviral vectors were used to infect Huh7 and Bel7402 cells, and the shRNA‐PRMT9 lentiviruses were used to infect SK‐Hep1 cells. Cells infected with pLV‐Puro‐GFP or PGCSIL‐GFP were used as controls. Infected cells were selected with 2 μg/mL puromycin for 2 weeks.