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Lysis buffer consisted of 980 μL Mammalian Protein Extraction Reagent (cat. no. 78503, ThermoFisher Scientific, www.thermofisher.com), 10 μL Halt Protease Inhibitor Cocktail (cat. no. 78415, Thermo Scientific), and 10 μL Halt Phosphatase Inhibitor Cocktail (cat. no. 78420, Thermo Scientific). The buffer was stored at -20°C prior to use.
Copyright and License information: ©2018 Gopinathan et al
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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